Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.7

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.2098	0.1786	0.1961
	B	25.00	0.1684	0.1633	0.1804
	C	8.33	0.1296	0.1325	0.1273
	D	2.78	0.08390	0.07235	0.07640
	E	0.93	0.04800	0.03750	0.03875
	F	0.31	0.01840	0.01460	0.01415
	G	0.10	0.006900	0.005250	0.005100
	H	0.03	0.003050	0.002250	0.002150

Yield (mg/mL): 1.7     Dilution Factor:  100×

Download these data

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by isaacmathews and last updated on 2023-11-29 12:00:34.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.001100	0.0006000	0.001400
	B	48.3	0.0008000	0.0007000
	C	45.7	0.001300		0.001200
	D	42.4	0.04820	0.04860	0.04990
	E	37.7	0.1937	0.1847	0.1843
	F	33.6	0.1940	0.1955	0.2040
	G	31.3	0.2268	0.2221	0.2095
	H	30.0	0.2272	0.2121	0.2062

Download these data

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by isaacmathews and last updated on 2023-11-29 12:06:49.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.