D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W409H

Expression Data

Yield (mg/mL): 2.872

Kinetic Assay Data

**slope of W409H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.40E-02	9.53E-02	9.65E-02
B	25.00	7.55E-02	8.43E-02	9.03E-02
C	8.33	6.96E-02	6.41E-02	6.17E-02
D	2.78	4.97E-02	4.53E-02	4.76E-02
E	0.93	2.90E-02	3.47E-02	2.66E-02
F	0.31	1.38E-02	1.34E-02	1.53E-02
G	0.10	6.15E-03	5.85E-03	4.65E-03
H	0.03	7.50E-04	6.00E-04	4.50E-04

Yield (mg/mL): 2.872 Dilution Factor: 100×

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by Yeisson and last updated on 2023-11-17 14:39:32.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.30E-01	3.29E-01	3.23E-01
B	25.00	2.88E-01	2.74E-01	2.75E-01
C	8.33	2.24E-01	2.14E-01	2.14E-01
D	2.78	1.28E-01	1.24E-01	1.27E-01
E	0.93	6.06E-02	5.64E-02	5.88E-02
F	0.31	2.19E-02	2.19E-02	2.24E-02
G	0.10	8.10E-03	8.10E-03	8.10E-03
H	0.03	0	0	0

Yield (mg/mL): 6.244 Dilution Factor: 100×

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by jrodr1379 and last updated on 2023-11-17 14:21:54.

Thermostability Assay Data

**slope of W409H A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	9.00E-04	9.00E-04
B	48.3		9.00E-04
C	45.7	9.00E-04		6.00E-04
D	42.4	8.55E-03	8.25E-03	7.80E-03
E	37.7	4.58E-02	4.79E-02	3.86E-02
F	33.6	5.43E-02	5.51E-02	4.98E-02
G	31.3	5.33E-02	5.69E-02	6.24E-02
H	30.0	5.63E-02	6.00E-02

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by Yeisson and last updated on 2023-11-17 15:00:25.

Reference Data for WT

**slope of W409H A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0	0
B	48.3	7.50E-04		6.00E-04
C	45.7	6.00E-04	6.00E-04	7.50E-04
D	42.4	1.83E-02	1.62E-02	1.62E-02
E	37.7	1.59E-01	1.54E-01	1.51E-01
F	33.6	1.88E-01	1.66E-01	1.72E-01
G	31.3	1.89E-01	1.61E-01	1.37E-01
H	30.0		1.42E-01	1.32E-01

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by jrodr1379 and last updated on 2023-11-17 14:27:37.

Design Data

Rosetta Score for W409H: 2.123

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.