D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W409T

Expression Data

Yield (mg/mL): 1.779

Kinetic Assay Data

**slope of W409T A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.35E-01	1.49E-01	2.11E-01
B	25.00	1.62E-01	1.11E-01	1.55E-01
C	8.33		1.52E-01	1.30E-01
D	2.78	9.51E-02	1.25E-01	1.16E-01
E	0.93	4.89E-02	5.55E-02	6.03E-02
F	0.31	2.16E-02	2.24E-02	2.36E-02
G	0.10	6.45E-03	8.70E-03	9.30E-03
H	0.03		0	0

Yield (mg/mL): 1.779 Dilution Factor: 100×

Protein purified on 11/3/2023 and assayed on 10/27/2023.
Data uploaded by dfern257 and last updated on 2023-11-17 14:55:36.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	9.02E-02	8.79E-02	9.23E-02
B	25.00	6.96E-02	7.31E-02	7.19E-02
C	8.33	4.73E-02	5.81E-02	6.23E-02
D	2.78		3.29E-02	3.57E-02
E	0.93	1.29E-02	1.67E-02	1.82E-02
F	0.31	6.00E-03	5.25E-03	5.55E-03
G	0.10	2.25E-03	2.25E-03	2.40E-03
H	0.03	6.00E-04	7.50E-04	6.00E-04

Yield (mg/mL): 2.821 Dilution Factor: 100×

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by jordangarcia0221 and last updated on 2023-11-17 14:13:26.

Thermostability Assay Data

**slope of W409T A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0		0
B	48.3	0	0	0
C	45.7	4.50E-04	7.50E-04	0
D	42.4	2.25E-03	2.10E-03	1.95E-03
E	37.7	3.93E-02	3.81E-02	3.26E-02
F	33.6	4.94E-02		4.59E-02
G	31.3	6.54E-02	7.29E-02	6.20E-02
H	30.0	7.23E-02	6.99E-02	6.87E-02

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by dfern257 and last updated on 2024-05-09 12:23:32.

Reference Data for WT

**slope of W409T A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	7.50E-04		7.50E-04
B	48.3	6.00E-04	4.50E-04
C	45.7	6.00E-04	6.00E-04	7.50E-04
D	42.4	4.95E-03	5.70E-03	4.95E-03
E	37.7	3.57E-02	3.35E-02	3.24E-02
F	33.6	3.86E-02	3.71E-02	3.45E-02
G	31.3	3.90E-02	3.45E-02	3.53E-02
H	30.0	3.06E-02	3.06E-02	2.78E-02

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by jordangarcia0221 and last updated on 2023-11-17 14:16:51.

Design Data

Rosetta Score for W409T: 3.084

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.