Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 2.821

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	9.02E-02	8.79E-02	9.23E-02
	B	25.00	6.96E-02	7.31E-02	7.19E-02
	C	8.33	4.73E-02	5.81E-02	6.23E-02
	D	2.78		3.29E-02	3.57E-02
	E	0.93	1.29E-02	1.67E-02	1.82E-02
	F	0.31	6.00E-03	5.25E-03	5.55E-03
	G	0.10	2.25E-03	2.25E-03	2.40E-03
	H	0.03	6.00E-04	7.50E-04	6.00E-04

Yield (mg/mL): 2.821     Dilution Factor:  100×

Download these data

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by jordangarcia0221 and last updated on 2023-11-17 14:13:26.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	7.50E-04		7.50E-04
	B	48.3	6.00E-04	4.50E-04
	C	45.7	6.00E-04	6.00E-04	7.50E-04
	D	42.4	4.95E-03	5.70E-03	4.95E-03
	E	37.7	3.57E-02	3.35E-02	3.24E-02
	F	33.6	3.86E-02	3.71E-02	3.45E-02
	G	31.3	3.90E-02	3.45E-02	3.53E-02
	H	30.0	3.06E-02	3.06E-02	2.78E-02

Download these data

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by jordangarcia0221 and last updated on 2023-11-17 14:16:51.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.