Raw Data for BglB Variant W409S

Expression Data

SDS-PAGE
 6.174

Kinetic Assay Data

slope of W409S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.72E-01 2.75E-01 2.64E-01
B 25.00 2.06E-01 2.07E-01 2.10E-01
C 8.33 1.53E-01 1.64E-01 1.14E-01
D 2.78 1.06E-01 1.19E-01 1.13E-01
E 0.93 6.18E-02 7.43E-02 6.45E-02
F 0.31 2.94E-02 2.88E-02 2.90E-02
G 0.10 1.16E-02 1.19E-02 1.07E-02
H 0.03 7.50E-04 6.00E-04
 6.174      100×

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by StephanieRuano and last updated on 2023-11-27 21:58:15.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.30E-01 3.29E-01 3.23E-01
B 25.00 2.88E-01 2.74E-01 2.75E-01
C 8.33 2.24E-01 2.14E-01 2.14E-01
D 2.78 1.28E-01 1.24E-01 1.27E-01
E 0.93 6.06E-02 5.64E-02 5.88E-02
F 0.31 2.19E-02 2.19E-02 2.24E-02
G 0.10 8.10E-03 8.10E-03 8.10E-03
H 0.03 0 0 0
 6.244      100×

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by jrodr1379 and last updated on 2023-11-17 14:21:54.


Thermostability Assay Data

slope of W409S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 0 0 0
C 45.7 1.20E-03 9.00E-04
D 42.4 5.70E-03 5.55E-03 5.70E-03
E 37.7 8.81E-02 9.60E-02 9.84E-02
F 33.6 9.36E-02 9.50E-02 9.54E-02
G 31.3 1.08E-01 1.00E-01 1.00E-01
H 30.0 1.00E-01 1.17E-01 1.12E-01

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by StephanieRuano and last updated on 2023-11-17 15:06:01.


Reference Data for WT

slope of W409S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 7.50E-04 6.00E-04
C 45.7 6.00E-04 6.00E-04 7.50E-04
D 42.4 1.83E-02 1.62E-02 1.62E-02
E 37.7 1.59E-01 1.54E-01 1.51E-01
F 33.6 1.88E-01 1.66E-01 1.72E-01
G 31.3 1.89E-01 1.61E-01 1.37E-01
H 30.0 1.42E-01 1.32E-01

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by jrodr1379 and last updated on 2023-11-17 14:27:37.


Design Data

4.119

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.