Raw Data for BglB Variant Y118F

Expression Data

Yield (mg/mL): 0.00945

Kinetic Assay Data

slope of Y118F A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00		6.60E-01	7.40E-01
	B	25.00	7.60E-01	7.70E-01	7.40E-01
	C	8.33	7.60E-01	7.20E-01	6.80E-01
	D	2.78	4.70E-01	4.90E-01	5.90E-01
	E	0.93	1.90E-01		2.10E-01
	F	0.31	6.00E-02	6.00E-02	6.00E-02
	G	0.10	2.00E-02	2.00E-02
	H	0.03	2.03E-03	1.99E-03	2.15E-03

Yield (mm): 0.00945     Dilution Factor:  10×

Download these data

Protein purified on 9/29/2023 and assayed on 10/13/2023.
Data uploaded by jornillo and last updated on 2023-11-17 11:31:27.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of Y118F A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.001997	0.003111	0.002726
	B	48.3	0.0033	0.002323	0.002897
	C	45.7	0.00384	0.001269	0.002254
	D	42.4	0.002829	0.002974
	E	37.7	0.00216	0.001869
	F	33.6	0.002734	0.002417
	G	31.3	0.002726	0.002074
	H	30.0	0.003883	0.001843	0.003197

Download these data

Protein purified on 9/29/2023 and assayed on 10/20/2023.
Data uploaded by jornillo and last updated on 2023-11-17 11:49:13.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.