D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant M114S

Yield (mg/mL): 0.04687

**slope of M114S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.36E-03	4.14E-03	2.73E-03
B	25.00	2.67E-03	4.29E-03
C	8.33	2.55E-03	2.97E-03
D	2.78	1.60E-03	2.43E-03
E	0.93	3.56E-03	2.61E-03	2.12E-03
F	0.31	1.77E-03	2.19E-03	1.17E-03
G	0.10	1.63E-03	2.67E-03	1.24E-03
H	0.03	1.96E-03	1.97E-03

Yield (mg/mL): 0.04687 Dilution Factor: 10×

Protein purified on 9/29/2023 and assayed on 10/13/2023.
Data uploaded by nking3 and last updated on 2023-11-17 11:02:37.

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

**slope of M114S A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		1.28E-03	1.19E-03
B	48.3		1.77E-03	1.93E-03
C	45.7		1.41E-03	1.58E-03
D	42.4	1.83E-03	1.77E-03
E	37.7	1.26E-03	1.35E-03
F	33.6	1.89E-03	2.19E-03	1.68E-03
G	31.3	2.08E-03	1.77E-03	1.58E-03
H	30.0		1.21E-03	1.07E-03

Protein purified on 9/29/2023 and assayed on 10/20/2023.
Data uploaded by nking3 and last updated on 2023-11-17 11:30:07.

**slope of M114S A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0.003994	0.002674	0.003514
B	48.3	0.002211	0.005409	0.003197
C	45.7	0.003746	0.004209	0.003566
D	42.4	0.03	0.03	0.03
E	37.7	0.05	0.05	0.05
F	33.6	0.06	0.06	0.05
G	31.3		0.06	0.06
H	30.0	0.07	0.07	0.08

Protein purified on 9/29/2023 and assayed on 10/20/2023.
Data uploaded by enielse and last updated on 2023-11-17 10:57:06.

Rosetta Score for M114S: 5.021

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.