D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Y118F

Yield (mg/mL): 0.4218

**slope of Y118F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.10E-01	1.30E-01	1.00E-01
B	25.00	8.00E-02	6.00E-02	7.00E-02
C	8.33	6.00E-02	5.00E-02	6.00E-02
D	2.78	4.00E-02	3.00E-02	4.00E-02
E	0.93	2.00E-02	2.00E-02	2.00E-02
F	0.31	7.27E-03	8.36E-03	8.61E-03
G	0.10	7.48E-03	4.28E-03	5.64E-03
H	0.03	1.85E-03		2.07E-03

Yield (mg/mL): 0.4218 Dilution Factor: 100×

Protein purified on 9/29/23 and assayed on 10/13/23.
Data uploaded by apatel2132 and last updated on 2023-12-01 13:12:27.

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

**slope of Y118F A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	30.0	2.31E-03	2.23E-03	1.92E-03
B	31.3	2.52E-03	2.38E-03
C	33.6	2.15E-03	1.60E-03	9.94E-04
D	37.7	1.62E-03	1.46E-03
E	42.4	1.18E-03	1.84E-03	2.63E-03
F	45.7	1.23E-03		9.26E-04
G	48.3	1.27E-03	1.73E-03	1.90E-03
H	50.0	1.88E-03	1.67E-03	1.65E-03

Protein purified on 9/29/23 and assayed on 10/20/23.
Data uploaded by apatel2132 and last updated on 2023-11-17 11:45:01.

**slope of Y118F A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0.003994	0.002674	0.003514
B	48.3	0.002211	0.005409	0.003197
C	45.7	0.003746	0.004209	0.003566
D	42.4	0.03	0.03	0.03
E	37.7	0.05	0.05	0.05
F	33.6	0.06	0.06	0.05
G	31.3		0.06	0.06
H	30.0	0.07	0.07	0.08

Protein purified on 9/29/2023 and assayed on 10/20/2023.
Data uploaded by enielse and last updated on 2023-11-17 10:57:06.

Rosetta Score for Y118F: 2.973

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.