Raw Data for BglB Variant C198K

Expression Data

SDS-PAGE
 1.759

Kinetic Assay Data

slope of C198K A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.20E-02 4.26E-02 4.13E-02
B 25.00 3.71E-02 3.66E-02 3.65E-02
C 8.33 2.63E-02 2.82E-02 2.69E-02
D 2.78 1.62E-02 1.59E-02 1.70E-02
E 0.93 7.05E-03 5.85E-03 8.55E-03
F 0.31 3.00E-03 3.00E-03 3.15E-03
G 0.10 1.35E-03 1.50E-03 1.20E-03
H 0.03 0 0
 1.759      100×

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by ahern798 and last updated on 2023-11-17 10:26:29.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.03195 0.05025
B 25.00 0.03330 0.04321
C 8.33 0.02295 0.01890
D 2.78 0.01620 0.01740
E 0.93 0.006000 0.007350 0.00570
F 0.31 0.001800 0.002250
G 0.10 0.0007500 0.001650 0.001350
H 0.03 0 0 0
 1.512      100×

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by ahern798 and last updated on 2024-05-09 14:21:31.


Thermostability Assay Data

slope of C198K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 0 0
C 45.7 9.00E-04 1.35E-03 6.00E-04
D 42.4 2.55E-03 1.95E-03 2.10E-03
E 37.7 1.19E-02 9.60E-03 9.45E-03
F 33.6 2.00E-02 1.64E-02 1.58E-02
G 31.3 7.95E-03 7.80E-03
H 30.0 1.04E-02 9.15E-03 8.85E-03

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by ahern798 and last updated on 2023-11-17 10:46:27.


Reference Data for WT

slope of C198K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 7.50E-04 7.50E-04
B 48.3 0 0 0
C 45.7 1.05E-03 7.50E-04 0
D 42.4 4.05E-03 3.45E-03 3.30E-03
E 37.7 1.76E-02 1.46E-02 2.01E-02
F 33.6 2.49E-02 2.40E-02 2.00E-02
G 31.3 2.55E-02 2.57E-02
H 30.0 2.61E-02 2.78E-02

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by ahern798 and last updated on 2023-11-17 10:49:46.


Design Data

55.567

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.