Raw Data for BglB Variant C198T

Expression Data

SDS-PAGE
 5.487

Kinetic Assay Data

slope of C198T A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.20E-01 2.26E-01 2.27E-01
B 25.00 1.60E-01 1.90E-01 1.97E-01
C 8.33 1.74E-01 1.61E-01 1.58E-01
D 2.78 1.11E-01 1.18E-01 1.25E-01
E 0.93 6.69E-02 6.20E-02 6.60E-02
F 0.31 3.30E-02 3.75E-02 3.24E-02
G 0.10 8.85E-03 9.45E-03 9.90E-03
H 0.03 0 0 0
 5.487      100×

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by JesusGentil and last updated on 2023-11-17 09:58:41.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.03195 0.05025
B 25.00 0.03330 0.04321
C 8.33 0.02295 0.01890
D 2.78 0.01620 0.01740
E 0.93 0.006000 0.007350 0.00570
F 0.31 0.001800 0.002250
G 0.10 0.0007500 0.001650 0.001350
H 0.03 0 0 0
 1.512      100×

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by ahern798 and last updated on 2024-05-09 14:21:31.


Thermostability Assay Data

slope of C198T A420 nm (min−1)
T (°C) 1 2 3
A 50.0 7.50E-04 7.50E-04 6.00E-04
B 48.3 6.00E-04 6.00E-04
C 45.7
D 42.4 1.35E-03 1.05E-03 1.20E-03
E 37.7 2.81E-02 2.48E-02 2.49E-02
F 33.6 8.58E-02 8.28E-02 8.72E-02
G 31.3 1.12E-01 1.01E-01 9.20E-02
H 30.0 9.86E-02 8.49E-02 8.01E-02

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by JesusGentil and last updated on 2024-05-09 11:36:10.


Reference Data for WT

slope of C198T A420 nm (min−1)
T (°C) 1 2 3
A 50.0 7.50E-04 7.50E-04
B 48.3 0 0 0
C 45.7 1.05E-03 7.50E-04 0
D 42.4 4.05E-03 3.45E-03 3.30E-03
E 37.7 1.76E-02 1.46E-02 2.01E-02
F 33.6 2.49E-02 2.40E-02 2.00E-02
G 31.3 2.55E-02 2.57E-02
H 30.0 2.61E-02 2.78E-02

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by ahern798 and last updated on 2023-11-17 10:49:46.


Design Data

3.153

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.