Raw Data for BglB Variant N404S

Expression Data

Kinetic Assay Data

Plate 20201217-4

slope of N404S A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.0130055	0.0111879	0.0121018
	B	18.75	0.00623515	0.00614848	0.00622
	C	4.69	0.00299152	0.00286242	0.00366848
	D	1.17	0.00143818	0.00168182	0.00169636
	E	0.29	0.0015	0.000973333	0.00243879
	F	0.07	0.000823636	0.000909091
	G	0.02	0.0010897	0.000723636
	H	0.00	0.00106727	0.000997576	0.00105273

Yield / A_{280 nm}: 4.957     Dilution Factor:  100×

Download these data

Protein purified on 9/27/19 and assayed on 9/27/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	8.86E-02	1.11E-01	8.31E-02
	B	18.75	7.30E-02	6.36E-02	5.65E-02
	C	4.69	5.06E-02	4.93E-02	4.65E-02
	D	1.17	2.51E-02	2.37E-02	2.22E-02
	E	0.29	9.62E-03	8.33E-03	8.61E-03
	F	0.07	2.82E-03	2.38E-03	2.40E-03
	G	0.02	7.90E-04	6.74E-04	7.26E-04
	H	0.00	1.88E-05	4.73E-05	0

Yield / A_{280 nm}: 1.03     Dilution Factor:  100×

Download these data

Protein purified on 9/21/19 and assayed on 9/21/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Citations

The above data have been published in the following journal article(s):

• • Huang, P.; Chu, S.K.; Frizzo, H.N.; Connolly, M.P.; Caster, R.W.; & Siegel, J. B. (2020) “Evaluating Protein Engineering Thermostability Prediction Tools Using an Independently Generated Dataset..” ACS Omega 5(12), 6487–6493.