Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 2.184

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	8.90E-02	8.75E-02	8.42E-02
	B	25.00	6.93E-02	7.55E-02	7.49E-02
	C	8.33	5.46E-02	5.99E-02	5.99E-02
	D	2.78	3.60E-02	3.69E-02	3.45E-02
	E	0.93	1.80E-02	1.70E-02	1.70E-02
	F	0.31	6.75E-03	7.65E-03	7.20E-03
	G	0.10		3.90E-03	3.60E-03
	H	0.03	1.95E-03	2.10E-03	1.65E-03

Yield (mg/mL): 2.184     Dilution Factor:  100×

Download these data

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by Sabrinargg and last updated on 2023-11-13 14:02:49.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	6.00E-04	0	9.00E-04
	B	48.3	0		0
	C	45.7	7.50E-04		7.50E-04
	D	42.4	4.35E-03	3.90E-03	3.00E-03
	E	37.7	3.63E-02	3.38E-02	3.15E-02
	F	35.6	3.47E-02	3.48E-02	3.48E-02
	G	31.3	4.74E-02	3.98E-02
	H	30.0	4.69E-02	4.31E-02	4.08E-02

Download these data

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by Sabrinargg and last updated on 2024-05-09 14:04:50.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.