Raw Data for BglB Variant N293I

Expression Data

SDS-PAGE
 0.6397

Kinetic Assay Data

slope of N293I A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.002400 0.002400 0.002550
B 25.00
C 8.33 0.002250 0.002399 0.002250
D 2.78 0.001350 0.001350 0.001350
E 0.93 0.001050 0.001050
F 0.31 0.0006000 0.0007500 0.0006000
G 0.10 0.0004500
H 0.03 0.0004500
 0.6397      10×

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by LizB and last updated on 2023-11-13 14:09:24.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.18E-01 2.41E-01 2.30E-01
B 25.00 1.72E-01 1.83E-01 1.84E-01
C 8.33 1.52E-01 1.59E-01 1.61E-01
D 2.78 9.78E-02 1.13E-01 9.99E-02
E 0.93 4.92E-02 5.99E-02 5.15E-02
F 0.31 2.21E-02 2.72E-02 2.54E-02
G 0.10 8.70E-03 1.26E-02 1.07E-02
H 0.03 1.65E-03 1.65E-03 1.65E-03
 5.041      100×

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by NicolasFuentes and last updated on 2023-11-13 14:06:10.


Thermostability Assay Data

slope of N293I A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.000000 0.0000000
B 48.3 0.0006000 0.0006000
C 45.7 0.0000000 0.0000000
D 42.4 0.001500 0.0009000 0.0000000
E 37.7 0.0007500 0.001050 0.0009000
F 33.6 0.001200 0.001350 0.001500
G 31.3 0.001350 0.001650 0.001200
H 30.0 0.001950 0.001350

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by LizB and last updated on 2023-11-13 14:21:08.


Reference Data for WT

slope of N293I A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0
B 48.3 1.05E-03 6.00E-04
C 45.7 1.05E-03 6.00E-04 7.50E-04
D 42.4 8.55E-03 8.55E-03
E 37.7 9.38E-02 1.03E-01 7.55E-02
F 33.6 1.12E-01 1.12E-01 8.67E-02
G 31.3 1.11E-01 1.11E-01 8.85E-02
H 30.0 1.13E-01 1.02E-01

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by NicolasFuentes and last updated on 2024-08-08 18:51:22.


Design Data

77.035

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.