D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N293S

Expression Data

Yield (mg/mL): 6.177

Kinetic Assay Data

**slope of N293S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.45E-03	6.30E-03	6.30E-03
B	25.00	4.65E-03	4.95E-03	5.10E-03
C	8.33	3.90E-03	4.05E-03	4.35E-03
D	2.78	2.40E-03	2.55E-03	2.25E-03
E	0.93	1.50E-03	1.50E-03	1.80E-03
F	0.31	9.00E-04	1.05E-03	1.05E-03
G	0.10	7.50E-04	7.50E-04	9.00E-04
H	0.03	4.50E-04	4.50E-04

Yield (mg/mL): 6.177 Dilution Factor: 100×

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by bryansouza and last updated on 2023-11-26 22:43:01.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.18E-01	2.41E-01	2.30E-01
B	25.00	1.72E-01	1.83E-01	1.84E-01
C	8.33	1.52E-01	1.59E-01	1.61E-01
D	2.78	9.78E-02	1.13E-01	9.99E-02
E	0.93	4.92E-02	5.99E-02	5.15E-02
F	0.31	2.21E-02	2.72E-02	2.54E-02
G	0.10	8.70E-03	1.26E-02	1.07E-02
H	0.03	1.65E-03	1.65E-03	1.65E-03

Yield (mg/mL): 5.041 Dilution Factor: 100×

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by NicolasFuentes and last updated on 2023-11-13 14:06:10.

Thermostability Assay Data

**slope of N293S A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0	7.50E-04
B	48.3	4.50E-04	6.00E-04	9.00E-04
C	45.7	6.00E-04		6.00E-04
D	42.4	7.50E-04	9.00E-04	9.00E-04
E	37.7	2.85E-03	2.55E-03	2.10E-03
F	33.6	2.55E-03	2.70E-03	2.10E-03
G	31.3	3.00E-03	2.55E-03	2.10E-03
H	30.0		2.40E-03	2.25E-03

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by LopezB and last updated on 2023-11-13 14:43:54.

Reference Data for WT

**slope of N293S A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0	0	0
B	48.3	1.05E-03	6.00E-04
C	45.7	1.05E-03	6.00E-04	7.50E-04
D	42.4	8.55E-03	8.55E-03
E	37.7	9.38E-02	1.03E-01	7.55E-02
F	33.6	1.12E-01	1.12E-01	8.67E-02
G	31.3	1.11E-01	1.11E-01	8.85E-02
H	30.0	1.13E-01	1.02E-01

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by NicolasFuentes and last updated on 2024-08-08 18:51:22.

Design Data

Rosetta Score for N293S: 0.699

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.