Raw Data for BglB Variant W325Q

Expression Data

SDS-PAGE
 1.427

Kinetic Assay Data

slope of W325Q A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.01290 0.01365 0.01290
B 25.00
C 8.33
D 2.78 0.01080 0.01260 0.01200
E 0.93 0.007950 0.002250 0.005400
F 0.31 0.003450 0.003300
G 0.10 0.001500 0.001650 0.001500
H 0.03 0.00E+00 0.00E+00
 1.427      10×

Protein purified on 10/30/2023 and assayed on 11/6/2023.
Data uploaded by FelixA and last updated on 2023-11-13 10:36:58.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.07410 0.07965 0.07845
B 25.00 0.07020 0.06135 0.06210
C 8.33 0.04620 0.04380 0.04830
D 2.78 0.02715 0.02970 0.02715
E 0.93 0.01620 0.01425 0.01245
F 0.31 0.006000 0.004950 0.005250
G 0.10 0.002850 0.001800 0.002250
H 0.03 0.0004500 0.001650 0.0000000
 1.677      100×

Protein purified on 10/30/2023 and assayed on 11/6/2023.
Data uploaded by kli023 and last updated on 2023-11-13 10:13:09.


Thermostability Assay Data

slope of W325Q A420 nm (min−1)
T (°C) 1 2 3
A 50.0 7.50E-04 6.00E-04 6.00E-04
B 48.3 7.50E-04 7.50E-04
C 45.7 6.00E-04 7.50E-04
D 42.4 9.00E-04 7.50E-04 7.50E-04
E 37.7 9.30E-03 9.60E-03 9.45E-03
F 33.6 1.17E-02 1.19E-02 1.19E-02
G 31.3 1.29E-02 1.25E-02 1.25E-02
H 30.0 1.17E-02 1.10E-02 1.07E-02

Protein purified on 10/30/2023 and assayed on 11/6/2023.
Data uploaded by FelixA and last updated on 2023-11-13 10:37:21.


Reference Data for WT

slope of W325Q A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0000000 0.0000000
B 48.3 0.0004500 0.0006000
C 45.7 0.0007500 0.0006000 0.0006000
D 42.4 0.002250 0.002250 0.001800
E 37.7 0.01710 0.01695 0.01605
F 33.6 0.02730 0.02805 0.02475
G 31.3 0.02865 0.02475 0.02535
H 30.0 0.02580 0.03060 0.02835

Protein purified on 10/30/2023 and assayed on 11/6/2023.
Data uploaded by kli023 and last updated on 2023-11-13 10:13:19.


Design Data

3.875

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.