D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T212E

Expression Data

Yield (mg/mL): 1.287

Kinetic Assay Data

**slope of T212E A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.12E-01	1.36E-01	1.42E-01
B	25.00	1.16E-01	1.18E-01	1.12E-01
C	8.33	8.78E-02	8.68E-02	8.40E-02
D	2.78	5.51E-02	4.96E-02	4.81E-02
E	0.93	2.42E-02	2.14E-02	2.10E-02
F	0.31	9.35E-03	7.70E-03	7.75E-03
G	0.10	3.40E-03	2.95E-03	2.90E-03
H	0.03	1.30E-03	1.10E-03	1.00E-03

Yield (mg/mL): 1.287 Dilution Factor: 100×

Protein purified on 10/19/23 and assayed on 10/23/23.
Data uploaded by mvlouie and last updated on 2023-10-26 15:50:29.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.59E-01	1.32E-01	1.27E-01
B	25.00	8.08E-02	9.99E-02	9.57E-02
C	8.33	7.24E-02	7.30E-02	7.36E-02
D	2.78	4.29E-02	4.40E-02	4.11E-02
E	0.93	1.86E-02	1.88E-02	1.73E-02
F	0.31	7.05E-03	7.10E-03	6.30E-03
G	0.10	2.45E-03	2.60E-03	2.40E-03
H	0.03	1.05E-03	1.05E-03	1.00E-03

Yield (mg/mL): 0.993 Dilution Factor: 100×

Protein purified on 10/19/23 and assayed on 10/23/23.
Data uploaded by mvlouie and last updated on 2023-10-26 13:56:26.

Design Data

Rosetta Score for T212E: -0.21

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.