D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N404M

Expression Data

Yield (mg/mL): 2.021

Kinetic Assay Data

**slope of N404M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0388	0.0402	0.03765
B	25.00	0.0205	0.0243	0.0275
C	8.33	0.0149	0.0163	0.01585
D	2.78	0.01025	0.0115	0.0086
E	0.93	0.00545	0.0057	0.0039
F	0.31	0.003	0.00285
G	0.10	0.00135	0.00135
H	0.03	0.00065	0.00075	0.0004

Yield (mg/mL): 2.021 Dilution Factor: 10×

Protein purified on 8/29/23 and assayed on 8/30/23.
Data uploaded by enniferj and last updated on 2023-10-10 17:05:34.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3006	0.2669	0.2839
B	25.00	0.239	0.2122	0.2238
C	8.33	0.1861	0.1664	0.1648
D	2.78	0.1088	0.1443	0.1156
E	0.93	0.06145		0.05445
F	0.31	0.0291		0.02455
G	0.10	0.0131		0.00975
H	0.03	0.00615		0.00355

Yield (mg/mL): 2.109 Dilution Factor: 100×

Protein purified on 8/29/23 and assayed on 8/30/23.
Data uploaded by enniferj and last updated on 2023-10-10 15:44:59.

Design Data

Rosetta Score for N404M: -2.964

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.