D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W409N

Expression Data

Yield (mg/mL): 4.934

Kinetic Assay Data

**slope of W409N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.18E-01	4.18E-01	4.31E-01
B	25.00	2.76E-01	2.70E-01	3.03E-01
C	8.33	2.16E-01	2.15E-01	2.12E-01
D	2.78	1.30E-01	1.31E-01	1.29E-01
E	0.93	6.08E-02	6.68E-02	6.80E-02
F	0.31	2.61E-02	2.52E-02	2.33E-02
G	0.10	6.95E-03	7.95E-03	8.75E-03
H	0.03	4.00E-04	3.50E-04	3.50E-04

Yield (mg/mL): 4.934 Dilution Factor: 100×

Protein purified on 9/25/23 and assayed on 9/26/23.
Data uploaded by mvlouie and last updated on 2023-10-01 15:05:09.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.81E-01	4.59E-01	4.69E-01
B	25.00	3.94E-01	4.13E-01	4.17E-01
C	8.33	2.56E-01	2.76E-01	2.72E-01
D	2.78	1.46E-01	1.64E-01	1.53E-01
E	0.93	5.79E-02	7.33E-02	5.55E-02
F	0.31	2.03E-02		1.97E-02
G	0.10	6.65E-03		6.70E-03
H	0.03	3.50E-04	3.00E-04	3.50E-04

Yield (mg/mL): 4.546 Dilution Factor: 100×

Protein purified on 9/25/23 and assayed on 9/26/23.
Data uploaded by mvlouie and last updated on 2023-09-30 17:12:13.

Design Data

Rosetta Score for W409N: 1.911

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.