D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F57Y

Expression Data

Yield (mg/mL): 2.75

Kinetic Assay Data

**slope of F57Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.04E-01	3.04E-01	3.01E-01
B	25.00	2.07E-01	2.35E-01	2.41E-01
C	8.33	1.97E-01	1.87E-01	1.78E-01
D	2.78	1.27E-01	1.04E-01	1.25E-01
E	0.93		4.40E-02	4.89E-02
F	0.31		1.57E-02	1.81E-02
G	0.10	1.13E-02	5.20E-03	7.10E-03
H	0.03		1.85E-03	2.25E-03

Yield (mg/mL): 2.75 Dilution Factor: 10×

Protein purified on 9/14/23 and assayed on 9/15/23.
Data uploaded by eLi and last updated on 2023-10-01 12:19:36.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.83E-01	3.90E-01	3.93E-01
B	25.00	3.29E-01	3.23E-01	3.23E-01
C	8.33	2.35E-01	2.27E-01	2.62E-01
D	2.78	1.28E-01	1.24E-01	1.68E-01
E	0.93	5.39E-02	5.34E-02
F	0.31	2.08E-02	2.01E-02
G	0.10	7.05E-03	6.90E-03
H	0.03	2.70E-03	2.25E-03

Yield (mg/mL): 1.364 Dilution Factor: 100×

Protein purified on 9/14/23 and assayed on 9/15/23.
Data uploaded by eLi and last updated on 2023-11-06 14:36:36.

Design Data

Rosetta Score for F57Y: -0.068

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.