D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Q276K

Expression Data

Yield (mg/mL): 0.736302

Kinetic Assay Data

**slope of Q276K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	7.24E-02	9.37E-02	8.52E-02
B	25.00	8.82E-02	9.21E-02	7.32E-02
C	8.33	6.64E-02	7.58E-02	6.83E-02
D	2.78	4.06E-02	3.98E-02	3.80E-02
E	0.93	1.74E-02	1.32E-02	1.55E-02
F	0.31	6.63E-03	6.74E-03	6.84E-03
G	0.10	2.59E-03	3.26E-03	3.47E-03
H	0.03	0	0

Yield (mg/mL): 0.736302 Dilution Factor: 100×

Protein purified on 2/29/2024 and assayed on 3/14/2024.
Data uploaded by sarahmullins and last updated on 2024-04-14 13:19:34.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.76E-01	2.90E-01	2.76E-01
B	25.00	2.19E-01	2.25E-01	2.22E-01
C	8.33	2.03E-01	1.96E-01	1.93E-01
D	2.78	1.16E-01	1.18E-01	1.26E-01
E	0.93	5.84E-02	5.35E-02	5.89E-02
F	0.31	2.34E-02	2.51E-02	2.54E-02
G	0.10	1.10E-02	1.13E-02	1.15E-02
H	0.03	0	0	0

Yield (mg/mL): 1.0257 Dilution Factor: 100×

Protein purified on 2/28/24 and assayed on 3/14/24.
Data uploaded by zizi197 and last updated on 2024-04-18 07:23:30.

Thermostability Assay Data

**slope of Q276K A_{420 nm} (min⁻¹)**
	T (°C)	1	2
A	30.0	3.51E-01	1.47E-01
B	30.5	1.56E-01	1.38E-01
C	31.7	2.64E-01	8.08E-02
D	33.2	0.18228	0.12569
E	35.4	0.15155	0.10003
F	38.3	0.041483	0.077793
G	41.7	0.024724	0.034862
H	44.5	0.0040345	0.0040345
I	46.8	0.0022759	0
J	48.3	0.0027931	0
K	49.3	0	0.0028966
L	50.0	0.003	0

Protein purified on 2/29/2024 and assayed on 3/21/2024.
Data uploaded by sarahmullins and last updated on 2024-04-14 13:21:26.

Reference Data for WT

**slope of Q276K A_{420 nm} (min⁻¹)**
	T (°C)	1	2
A	30.0	2.68E-01	2.43E-01
B	30.5	2.24E-01	2.30E-01
C	31.7	2.60E-01	2.47E-01
D	33.2	0.22397	0.2219
E	35.4	0.20503	0.23079
F	38.3	0.24248	0.21724
G	41.7	0.087103	0.097345
H	44.5	0.0058966	0.0066207
I	46.8	0	0.0028966
J	48.3	0	0.0024828
K	49.5	0	0
L	50.0	0	0

Protein purified on 2/28/24 and assayed on 3/21/24.
Data uploaded by zizi197 and last updated on 2024-04-11 07:12:47.

Design Data

Rosetta Score for Q276K: 4.566

Citations

The unpublished data above were collected by students from Taylor University. For inquiries, please contact Daniel Kaluka: <daniel_kaluka@taylor.edu>.