Raw Data for BglB Variant W120Y

Expression Data

SDS-PAGE
 2.366

Kinetic Assay Data

slope of W120Y A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 8.20E-03 8.63E-03 8.55E-03
B 25.00 6.58E-03 6.80E-03 6.63E-03
C 8.33 2.70E-03 2.28E-03 2.15E-03
D 2.78 6.75E-04 1.00E-03 7.75E-04
E 0.93 4.50E-04 4.75E-04 4.50E-04
F 0.31 2.75E-04 3.25E-04
G 0.10 0.00E+00 0.00E+00 0.00E+00
H 0.03 0.00E+00 0.00E+00
 2.366      10×

Protein purified on 7/6/23 and assayed on 7/7/23.
Data uploaded by alexasvf and last updated on 2023-07-27 01:38:54.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.87E-01 3.82E-01 3.95E-01
B 25.00 3.27E-01 3.23E-01 3.24E-01
C 8.33 2.11E-01 2.00E-01 2.06E-01
D 2.78 9.90E-02 1.02E-01 1.18E-01
E 0.93 4.35E-02 4.32E-02 4.98E-02
F 0.31 1.35E-02 1.45E-02 1.64E-02
G 0.10 5.40E-03 4.95E-03 5.80E-03
H 0.03 0 0
 3.88      100×

Protein purified on 7/6/23 and assayed on 7/7/23.
Data uploaded by alexasvf and last updated on 2023-07-27 01:11:37.



Design Data

11.123


Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.