D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H315P

Expression Data

Yield (mg/mL): 0.841878

Kinetic Assay Data

**slope of H315P A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		0.15486	0.15703
B	25.00	0.126	0.13003
C	8.33	0.092172	0.096517	0.097448
D	2.78	0.053379	0.058655	0.06031
E	0.93	0.025345	0.026379	0.026793
F	0.31	0.0095593	0.010448	0.010345
G	0.10	0.0035593	0.003931	0.0042414
H	0.03	0	0	0

Yield (mg/mL): 0.841878 Dilution Factor: 100×

Protein purified on 7/17/23 and assayed on 7/18/23.
Data uploaded by annikabennett and last updated on 2023-07-21 10:27:41.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.34E-01	3.66E-01	3.54E-01
B	25.00	2.75E-01	2.88E-01	3.00E-01
C	8.33	2.20E-01	2.30E-01	2.12E-01
D	2.78	1.26E-01	1.29E-01	1.29E-01
E	0.93	5.72E-02	5.99E-02	5.76E-02
F	0.31	2.19E-02	2.37E-02	2.21E-02
G	0.10	8.03E-03	8.28E-03	7.76E-03
H	0.03	0	0	0

Yield (mg/mL): 1.638 Dilution Factor: 100×

Protein purified on 7/17/2023 and assayed on 7/18/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-21 08:26:19.

Thermostability Assay Data

**slope of H315P A_{420 nm} (min⁻¹)**
	T (°C)	1	2
A	30.0	0.20814	0.21817
B	30.5	0.20266
C	31.7	0.1921	0.18507
D	33.2	0.18031	0.18507
E	35.4	0.15869	0.16521
F	38.3	0.13438	0.13179
G	41.7	0.043138	0.041172
H	44.5	0.0047586	0.0025862
I	46.8	0.0041379	0.0028966
J	48.3	0.002069	0.0034138
K	49.5	0.0022759	0.0033103
L	50.0	0.0052759

Protein purified on 7/17/23 and assayed on 7/19/23.
Data uploaded by annikabennett and last updated on 2023-07-21 10:31:59.

Reference Data for WT

**slope of H315P A_{420 nm} (min⁻¹)**
	T (°C)	1	2
A	30.0	4.67E-01	4.79E-01
B	30.5	4.15E-01	3.08E-01
C	31.7	4.62E-01	4.63E-01
D	33.2	0.43976	0.40645
E	35.4		0.41514
F	38.3	0.33538	0.32938
G	41.7		0.099828
H	44.5	0.0054828	0.0056897
I	46.8	0.0013448	0.0024828
J	48.3	0.0028966	0.0010345
K	49.5	0.0032069	0.030724
L	50.0	0.0025862	0.0017586

Protein purified on 7/17/2023 and assayed on 7/19/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-21 10:15:59.

Design Data

Rosetta Score for H315P: -1.849

Citations

The unpublished data above were collected by students from Taylor University. For inquiries, please contact Daniel Kaluka: <daniel_kaluka@taylor.edu>.