D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F243H

Expression Data

Yield (mg/mL): 1.5745

Kinetic Assay Data

**slope of F243H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.08E-01	1.13E-01	1.09E-01
B	25.00	8.27E-02	9.13E-02	8.23E-02
C	8.33	6.46E-02	6.93E-02	6.79E-02
D	2.78	4.73E-02	4.29E-02	4.16E-02
E	0.93	1.94E-02	2.04E-02	1.91E-02
F	0.31	8.38E-03	8.69E-03	7.86E-03
G	0.10	4.66E-03	5.17E-03	5.17E-03
H	0.03	2.79E-03		2.17E-03

Yield (mg/mL): 1.5745 Dilution Factor: 100×

Protein purified on 7/17/2023 and assayed on 7/18/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-21 08:39:24.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.34E-01	3.66E-01	3.54E-01
B	25.00	2.75E-01	2.88E-01	3.00E-01
C	8.33	2.20E-01	2.30E-01	2.12E-01
D	2.78	1.26E-01	1.29E-01	1.29E-01
E	0.93	5.72E-02	5.99E-02	5.76E-02
F	0.31	2.19E-02	2.37E-02	2.21E-02
G	0.10	8.03E-03	8.28E-03	7.76E-03
H	0.03	0	0	0

Yield (mg/mL): 1.638 Dilution Factor: 100×

Protein purified on 7/17/2023 and assayed on 7/18/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-21 08:26:19.

Thermostability Assay Data

**slope of F243H A_{420 nm} (min⁻¹)**
	T (°C)	1	2
A	30.0	3.87E-01	4.07E-01
B	30.5	3.68E-01	3.80E-01
C	31.7	3.69E-01	3.61E-01
D	33.2	0.34883	0.27703
E	35.4	0.32566	0.30197
F	38.3	0.28903	0.27972
G	41.7	0.090931	0.11545
H	44.5	0.015931	0.0056897
I	46.8	0.0031034	0.0033103
J	48.3	0.0022759	0
K	49.5	0	0
L	50.0	0.0011379	0.0022759

Protein purified on 7/17/2023 and assayed on 7/19/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-21 08:47:19.

Reference Data for WT

**slope of F243H A_{420 nm} (min⁻¹)**
	T (°C)	1	2
A	30.0	4.67E-01	4.79E-01
B	30.5	4.15E-01	3.08E-01
C	31.7	4.62E-01	4.63E-01
D	33.2	0.43976	0.40645
E	35.4		0.41514
F	38.3	0.33538	0.32938
G	41.7		0.099828
H	44.5	0.0054828	0.0056897
I	46.8	0.0013448	0.0024828
J	48.3	0.0028966	0.0010345
K	49.5	0.0032069	0.030724
L	50.0	0.0025862	0.0017586

Protein purified on 7/17/2023 and assayed on 7/19/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-21 10:15:59.

Design Data

Rosetta Score for F243H: 1.559

Citations

The unpublished data above were collected by students from Taylor University. For inquiries, please contact Daniel Kaluka: <daniel_kaluka@taylor.edu>.