Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.63825

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.05E-01	3.26E-01	3.35E-01
	B	25.00	2.62E-01	2.82E-01	3.00E-01
	C	8.33	2.08E-01	2.31E-01	2.11E-01
	D	2.78	1.22E-01	1.32E-01	1.23E-01
	E	0.93	5.61E-02	5.47E-02	6.32E-02
	F	0.31	2.19E-02	2.15E-02	2.18E-02
	G	0.10	8.69E-03	8.07E-03	9.21E-03
	H	0.03	0		0

Yield (mg/mL): 1.63825     Dilution Factor:  100×

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Protein purified on 7/17/2023 and assayed on 7/18/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-20 11:00:12.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2
	A	30.0	4.95E-01	5.40E-01
	B	30.5	4.96E-01	5.05E-01
	C	31.7	5.25E-01	5.23E-01
	D	33.2	0.47234	0.50421
	E	35.4	0.46055	0.48352
	F	38.3	0.40634	0.41586
	G	41.7	0.10655	0.11048
	H	44.5	0.0046552	0.0049655
	I	46.8	0	0
	J	48.3	0	0
	K	49.5	0	0
	L	50.0	0	0

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Protein purified on 7/17/2023 and assayed on 7/19/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-20 11:04:31.

Citations

The unpublished data above were collected by students from Taylor University. For inquiries, please contact Daniel Kaluka: <daniel_kaluka@taylor.edu>.