D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220A

Expression Data

Yield (mg/mL): 0.588

Kinetic Assay Data

**slope of N220A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.93E-01	2.06E-01	2.05E-01
B	25.00	1.61E-01	1.61E-01	1.54E-01
C	8.33	1.02E-01	1.05E-01	1.10E-01
D	2.78	3.85E-02	3.90E-02	4.01E-02
E	0.93	1.51E-02	1.36E-02	1.45E-02
F	0.31	5.15E-03	4.50E-03	4.95E-03
G	0.10	1.90E-03	1.45E-03	1.90E-03
H	0.03	7.50E-04	7.00E-04	9.00E-04

Yield (mg/mL): 0.588 Dilution Factor: 10×

Protein purified on 7/12/2023 and assayed on 7/13/2023.
Data uploaded by kaschilling and last updated on 2023-07-13 20:07:18.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.88E-02		4.67E-02
B	25.00	4.34E-02	3.86E-02	4.05E-02
C	8.33	4.19E-02	3.98E-02	3.41E-02
D	2.78	1.96E-02	2.11E-02	2.51E-02
E	0.93	1.74E-02	1.66E-02	1.54E-02
F	0.31	0		0
G	0.10
H	0.03

Yield (mg/mL): 0.652 Dilution Factor: 100×

Protein purified on 7/12/2023 and assayed on 7/13/2023.
Data uploaded by kaschilling and last updated on 2023-07-13 19:48:53.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of N220A A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0
B	48.3
C	45.7	1.30E-03	1.00E-03	1.30E-03
D	42.4	1.74E-02	1.41E-02	1.56E-02
E	37.7		5.04E-02
F	33.6		5.34E-02
G	31.3	5.05E-02	4.31E-02	5.47E-02
H	30.0	4.98E-02	5.24E-02	4.98E-02

Protein purified on 7/12/2023 and assayed on 7/13/2023.
Data uploaded by kaschilling and last updated on 2023-07-13 19:56:52.

Design Data

Rosetta Score for N220A: 1.721

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Kate Schilling: <katherine.schilling@yale.edu>.