Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.652

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	5.10E-02	4.34E-02	4.95E-02
	B	25.00	4.64E-02	4.90E-02	4.89E-02
	C	8.33	3.99E-02	3.87E-02	3.44E-02
	D	2.78	2.07E-02	2.01E-02	1.98E-02
	E	0.93	1.04E-02	8.90E-03	9.45E-03
	F	0.31	4.10E-03	3.65E-03	3.55E-03
	G	0.10	1.70E-03	4.00E-04	1.35E-03
	H	0.03		7.50E-04	6.00E-04

Yield (mg/mL): 0.652     Dilution Factor:  100×

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Protein purified on 7/12/23 and assayed on 7/13/23.
Data uploaded by hrmalcolm and last updated on 2023-07-13 19:57:37.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	6.00E-04	4.00E-04	8.00E-04
	B	48.3		7.00E-04	9.00E-04
	C	45.7	1.30E-03	1.00E-03	1.30E-03
	D	42.4	1.74E-02	1.41E-02	1.56E-02
	E	37.7	4.37E-02	5.04E-02	3.50E-02
	F	33.6	4.51E-02	5.34E-02	3.83E-02
	G	31.3	5.05E-02	4.31E-02	5.47E-02
	H	30.0	4.98E-02	5.24E-02	4.98E-02

Download these data

Protein purified on 7/12/2023 and assayed on 7/13/2023.
Data uploaded by hrmalcolm and last updated on 2023-07-13 20:02:40.

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.