D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E340S

Expression Data

Yield (mg/mL): 0.812

Kinetic Assay Data

**slope of E340S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.02E-02	6.77E-02	6.83E-02
B	25.00	6.56E-02	6.66E-02	7.34E-02
C	8.33	5.63E-02	5.78E-02	6.29E-02
D	2.78	2.76E-02	2.85E-02	3.11E-02
E	0.93	1.14E-02	1.24E-02	1.17E-02
F	0.31	4.95E-03	5.20E-03	5.00E-03
G	0.10	2.10E-03	1.95E-03	1.75E-03
H	0.03	7.00E-04	9.50E-04	7.00E-04

Yield (mg/mL): 0.812 Dilution Factor: 100×

Protein purified on 7/12/23 and assayed on 7/13/23.
Data uploaded by hrmalcolm and last updated on 2023-07-13 19:42:11.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.10E-02	4.34E-02	4.95E-02
B	25.00	4.64E-02	4.90E-02	4.89E-02
C	8.33	3.99E-02	3.87E-02	3.44E-02
D	2.78	2.07E-02	2.01E-02	1.98E-02
E	0.93	1.04E-02	8.90E-03	9.45E-03
F	0.31	4.10E-03	3.65E-03	3.55E-03
G	0.10	1.70E-03	4.00E-04	1.35E-03
H	0.03		7.50E-04	6.00E-04

Yield (mg/mL): 0.652 Dilution Factor: 100×

Protein purified on 7/12/23 and assayed on 7/13/23.
Data uploaded by hrmalcolm and last updated on 2023-07-13 19:57:37.

Design Data

Rosetta Score for E340S: 4.417

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.