D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H223A

Yield (mg/mL): 0.49

**slope of H223A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		0.03280	0.03340
B	25.00	0.02870	0.03205	0.03335
C	8.33	0.02320	0.02155	0.02595
D	2.78	0.01525	0.01410	0.01560
E	0.93	0.01130	0.01230	0.01225
F	0.31
G	0.10
H	0.03

Yield (mg/mL): 0.49 Dilution Factor: 100×

Protein purified on 7/12/2023 and assayed on 7/13/2023.
Data uploaded by jjones and last updated on 2023-07-13 19:57:24.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.88E-02		4.67E-02
B	25.00	4.34E-02	3.86E-02	4.05E-02
C	8.33	4.19E-02	3.98E-02	3.41E-02
D	2.78	1.96E-02	2.11E-02	2.51E-02
E	0.93	1.74E-02	1.66E-02	1.54E-02
F	0.31	0		0
G	0.10
H	0.03

Yield (mg/mL): 0.652 Dilution Factor: 100×

Protein purified on 7/12/2023 and assayed on 7/13/2023.
Data uploaded by jjones and last updated on 2023-07-13 20:00:22.

**slope of H223A A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0.0006000	0.0004001	0.0000000
B	48.3	0.0005000	0.0006000
C	45.7	0.0008000	0.0009000	0.0007000
D	42.4	0.003700	0.003100
E	37.7	0.009500	0.008800
F	33.6		0.008500	0.006600
G	31.3		0.009900	0.009000
H	30.0	0.02010	0.01250	0.009000

Protein purified on 7/12/2023 and assayed on 7/13/2023.
Data uploaded by jjones and last updated on 2023-07-13 20:27:42.

**slope of H223A A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	4.00E-04	8.00E-04
B	48.3		7.00E-04	9.00E-04
C	45.7	1.30E-03	1.00E-03	1.30E-03
D	42.4	1.74E-02	1.41E-02	1.56E-02
E	37.7	4.37E-02	5.04E-02	3.50E-02
F	33.6	4.51E-02	5.34E-02	3.83E-02
G	31.3	5.05E-02	4.31E-02	5.47E-02
H	30.0	4.98E-02	5.24E-02	4.98E-02

Protein purified on 7/12/2023 and assayed on 7/13/2023.
Data uploaded by jjones and last updated on 2023-07-13 20:08:52.

Rosetta Score for H223A: -2.501

The unpublished data above were collected by students from La Sierra University. For inquiries, please contact Jesica Jones: <jjones1@lasierra.edu>.