D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N404L

Expression Data

Yield (mg/mL): 0.938

Kinetic Assay Data

**slope of N404L A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.95E-02	6.00E-02	6.26E-02
B	25.00	3.76E-02	3.80E-02	3.93E-02
C	8.33	1.78E-02	1.72E-02	1.77E-02
D	2.78	5.35E-03	5.95E-03	6.15E-03
E	0.93	2.25E-03	1.95E-03	1.90E-03
F	0.31	9.50E-04	7.00E-04	8.50E-04
G	0.10	5.50E-04	4.50E-04	5.00E-04
H	0.03	4.00E-04	3.00E-04	3.50E-04

Yield (mg/mL): 0.938 Dilution Factor: 10×

Protein purified on 7/12/23 and assayed on 7/13/23.
Data uploaded by hrmalcolm and last updated on 2023-07-13 19:38:09.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.10E-02	4.34E-02	4.95E-02
B	25.00	4.64E-02	4.90E-02	4.89E-02
C	8.33	3.99E-02	3.87E-02	3.44E-02
D	2.78	2.07E-02	2.01E-02	1.98E-02
E	0.93	1.04E-02	8.90E-03	9.45E-03
F	0.31	4.10E-03	3.65E-03	3.55E-03
G	0.10	1.70E-03	4.00E-04	1.35E-03
H	0.03		7.50E-04	6.00E-04

Yield (mg/mL): 0.652 Dilution Factor: 100×

Protein purified on 7/12/23 and assayed on 7/13/23.
Data uploaded by hrmalcolm and last updated on 2023-07-13 19:57:37.

Design Data

Rosetta Score for N404L: -1.537

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.