Raw Data for BglB Variant V398N

Expression Data

SDS-PAGE
 0.406832

Kinetic Assay Data

slope of V398N A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.045 0.047483 0.04769
B 25.00 0.040241 0.041897 0.03869
C 8.33 0.033828 0.032069 0.031966
D 2.78 0.019138 0.019034 0.019448
E 0.93 0.0086897 0.0093103 0.0079655
F 0.31 0.0043448 0.0051724 0.005069
G 0.10 0.0028966 0.003 0.0035172
H 0.03 0 0
 0.406832      100×

Protein purified on 7/17/23 and assayed on 7/18/23.
Data uploaded by annikabennett and last updated on 2023-07-20 11:14:34.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.05E-01 3.26E-01 3.35E-01
B 25.00 2.62E-01 2.82E-01 3.00E-01
C 8.33 2.08E-01 2.31E-01 2.11E-01
D 2.78 1.22E-01 1.32E-01 1.23E-01
E 0.93 5.61E-02 5.47E-02 6.32E-02
F 0.31 2.19E-02 2.15E-02 2.18E-02
G 0.10 8.69E-03 8.07E-03 9.21E-03
H 0.03 0 0
 1.63825      100×

Protein purified on 7/17/2023 and assayed on 7/18/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-20 11:00:12.


Thermostability Assay Data

slope of V398N A420 nm (min−1)
T (°C) 1 2
A 30.0 0.045828 0.052552
B 30.5 0.047172 0.042621
C 31.7 0.041172 0.037655
D 33.2 0.027 0.025138
E 35.4 0.018931 0.016759
F 38.3 0.0092069 0.0081724
G 41.7 0.0025862 0.003
H 44.5 0 0
I 46.8 0 0
J 48.3 0 0
K 49.5 0 0
L 50.0 0 0

Protein purified on 7/17/23 and assayed on 7/19/23.
Data uploaded by annikabennett and last updated on 2023-07-20 11:42:51.


Reference Data for WT

slope of V398N A420 nm (min−1)
T (°C) 1 2
A 30.0 4.95E-01 5.40E-01
B 30.5 4.96E-01 5.05E-01
C 31.7 5.25E-01 5.23E-01
D 33.2 0.47234 0.50421
E 35.4 0.46055 0.48352
F 38.3 0.40634 0.41586
G 41.7 0.10655 0.11048
H 44.5 0.0046552 0.0049655
I 46.8 0 0
J 48.3 0 0
K 49.5 0 0
L 50.0 0 0

Protein purified on 7/17/2023 and assayed on 7/19/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-20 11:04:31.


Design Data

4.58

Citations

The unpublished data above were collected by students from Taylor University. For inquiries, please contact Daniel Kaluka: <daniel_kaluka@taylor.edu>.