D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E26K

Expression Data

Yield (mg/mL): 0.623445

Kinetic Assay Data

**slope of E26K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.12E-01	1.15E-01	1.10E-01
B	25.00	8.70E-02	8.83E-02	8.84E-02
C	8.33	6.09E-02	6.29E-02	6.46E-02
D	2.78	3.77E-02	3.76E-02	3.66E-02
E	0.93	1.71E-02	1.70E-02	1.75E-02
F	0.31	6.62E-03	7.34E-03	6.93E-03
G	0.10	5.28E-03	4.97E-03
H	0.03	0	3.21E-03	2.17E-03

Yield (mg/mL): 0.623445 Dilution Factor: 100×

Protein purified on 7/17/2023 and assayed on 7/18/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-20 09:13:32.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.05E-01	3.26E-01	3.35E-01
B	25.00	2.62E-01	2.82E-01	3.00E-01
C	8.33	2.08E-01	2.31E-01	2.11E-01
D	2.78	1.22E-01	1.32E-01	1.23E-01
E	0.93	5.61E-02	5.47E-02	6.32E-02
F	0.31	2.19E-02	2.15E-02	2.18E-02
G	0.10	8.69E-03	8.07E-03	9.21E-03
H	0.03	0		0

Yield (mg/mL): 1.63825 Dilution Factor: 100×

Protein purified on 7/17/2023 and assayed on 7/18/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-20 11:00:12.

Thermostability Assay Data

**slope of E26K A_{420 nm} (min⁻¹)**
	T (°C)	1	2
A	30.0	1.63E-01	1.61E-01
B	30.5	1.56E-01	1.48E-01
C	31.7	1.57E-01	1.59E-01
D	33.2	0.14576	0.14162
E	35.4		0.1319
F	38.3	0.10531	0.061655
G	41.7	0.034448	0.026586
H	44.5	0.0032069	0.003
I	46.8	0	0
J	48.3	0.0022759	0.024103
K	49.5	0	0
L	50.0	0	0.020897

Protein purified on 7/17/2023 and assayed on 7/19/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-21 10:52:08.

Reference Data for WT

**slope of E26K A_{420 nm} (min⁻¹)**
	T (°C)	1	2
A	30.0	4.95E-01	5.40E-01
B	30.5	4.96E-01	5.05E-01
C	31.7	5.25E-01	5.23E-01
D	33.2	0.47234	0.50421
E	35.4	0.46055	0.48352
F	38.3	0.40634	0.41586
G	41.7	0.10655	0.11048
H	44.5	0.0046552	0.0049655
I	46.8	0	0
J	48.3	0	0
K	49.5	0	0
L	50.0	0	0

Protein purified on 7/17/2023 and assayed on 7/19/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-20 11:04:31.

Design Data

Rosetta Score for E26K: -0.905

Citations

The unpublished data above were collected by students from Taylor University. For inquiries, please contact Daniel Kaluka: <daniel_kaluka@taylor.edu>.