Raw Data for BglB Variant N220W

Expression Data

Kinetic Assay Data

Plate 20201217-3

slope of N220W A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.00462	0.00477
	B	18.75	0.00387	0.00375
	C	4.69	0.00337	0.0035
	D	1.17	0.00297	0.0029	0.00208
	E	0.29	0.00193	0.00177	0.00136
	F	0.07	0.00078	0.00069	0.00064
	G	0.02	0.00021	0.00026	0.00025
	H	0.00		0.00006	0.00005

Yield / A_{280 nm}: 0.581     Dilution Factor:  100×

Download these data

Protein purified on 9/30/19 and assayed on 9/30/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	6.11E-02	6.35E-02	4.72E-02
	B	18.75	4.82E-02	3.18E-02	3.96E-02
	C	4.69	2.79E-02	2.73E-02	2.27E-02
	D	1.17	1.22E-02	1.14E-02	1.01E-02
	E	0.29	3.87E-03	3.52E-03	3.36E-03
	F	0.07	1.10E-03	1.00E-03	9.44E-04
	G	0.02	2.80E-04	2.51E-04	3.53E-04
	H	0.00	0	0	0

Yield / A_{280 nm}: 1.155     Dilution Factor:  100×

Download these data

Protein purified on 9/30/19 and assayed on 9/30/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Citations

The above data have been published in the following journal article(s):

• • Huang, P.; Chu, S.K.; Frizzo, H.N.; Connolly, M.P.; Caster, R.W.; & Siegel, J. B. (2020) “Evaluating Protein Engineering Thermostability Prediction Tools Using an Independently Generated Dataset..” ACS Omega 5(12), 6487–6493.