D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E154A

Expression Data

Yield (mg/mL): 0.27

Kinetic Assay Data

**slope of E154A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.85E-03	6.20E-03	6.25E-03
B	25.00	4.30E-03	5.30E-03	5.10E-03
C	8.33	4.00E-04		4.50E-04
D	2.78
E	0.93
F	0.31
G	0.10	5.00E-04	4.00E-04	5.00E-04
H	0.03	0	0	0

Yield (mg/mL): 0.27 Dilution Factor: 100×

Protein purified on 5/17/23 and assayed on 5/24/23.
Data uploaded by eLi and last updated on 2023-06-13 20:14:44.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.51E-01	2.43E-01	2.50E-01
B	25.00	1.97E-01	1.81E-01	1.88E-01
C	8.33
D	2.78
E	0.93	4.21E-02	4.13E-02	4.13E-02
F	0.31	1.32E-02	1.10E-02	1.06E-02
G	0.10	3.05E-03		2.80E-03
H	0.03		2.60E-03	2.40E-03

Yield (mg/mL): 1.6 Dilution Factor: 100×

Protein purified on 5/17/23 and assayed on 5/24/23.
Data uploaded by eLi and last updated on 2023-05-31 10:28:58.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of E154A A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	0	4.00E-04
B	48.3	0	0
C	45.7	1.00E-03	1.00E-03	9.00E-04
D	42.4	1.35E-02	1.26E-02	1.15E-02
E	37.7	5.94E-02	5.81E-02	5.47E-02
F	33.6		6.24E-02	5.23E-02
G	31.3	5.97E-02	6.21E-02	6.54E-02
H	30.0	5.93E-02	6.38E-02	6.48E-02

Protein purified on 5/17/23 and assayed on 5/24/23.
Data uploaded by eLi and last updated on 2023-05-31 10:37:46.

Design Data

Rosetta Score for E154A: 1.597

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.