Raw Data for BglB Variant W264A

Expression Data

Yield (mg/mL): 0.44

Kinetic Assay Data

slope of W264A A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.49E-03	1.16E-03	1.19E-03
	B	25.00	1.26E-03	1.50E-03	1.31E-03
	C	8.33	1.08E-03	1.05E-03	1.05E-03
	D	2.78	7.50E-04	7.71E-04
	E	0.93	4.90E-04	5.65E-04	5.07E-04
	F	0.31		4.10E-04	3.80E-04
	G	0.10	3.98E-04	4.21E-04
	H	0.03	2.63E-04	2.42E-04	3.39E-04

Yield (mg/mL): 0.44     Dilution Factor:  10×

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Protein purified on 5/17/23 and assayed on 5/26/23.
Data uploaded by atlopez and last updated on 2023-06-02 20:48:14.

Thermostability Assay Data

slope of W264A A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	3.00E-04	7.00E-04	5.00E-04
	B	48.3	5.00E-04	7.00E-04	6.00E-04
	C	45.7	4.00E-04	4.00E-04
	D	42.4	4.00E-04	5.00E-04	7.00E-04
	E	37.7	6.00E-04	6.00E-04
	F	33.6	8.00E-04	7.00E-04	7.00E-04
	G	31.3	8.00E-04	6.00E-04	7.00E-04
	H	30.0	1.00E-03	8.00E-04	4.00E-04

Download these data

Protein purified on 5/17/2023 and assayed on 5/27/2023.
Data uploaded by atlopez and last updated on 2023-06-02 21:47:16.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.