D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W325A

Expression Data

Yield (mg/mL): 0.306

Kinetic Assay Data

**slope of W325A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.17E-01	1.21E-01	1.18E-01
B	25.00	8.10E-02	7.00E-02	7.00E-02
C	8.33	5.00E-02	4.90E-02	5.30E-02
D	2.78	4.40E-02	4.90E-02	4.20E-02
E	0.93	4.40E-02	4.30E-02	4.10E-02
F	0.31	5.00E-02	4.20E-02	4.30E-02
G	0.10	4.00E-02	4.20E-02	4.00E-02
H	0.03	4.10E-02	4.30E-02	4.90E-02

Yield (mg/mL): 0.306 Dilution Factor: 10×

Protein purified on 5/17/2023 and assayed on 5/23/2023.
Data uploaded by NhuBui and last updated on 2023-05-31 13:57:45.

Thermostability Assay Data

**slope of W325A A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0
B	48.3	0	0	0
C	45.7	0	0	0
D	42.4	0	0	0
E	37.7		0	0
F	33.6	0		0
G	31.3		6.00E-04	5.00E-04
H	30.0	5.00E-04	4.00E-04

Protein purified on 5/17/2023 and assayed on 5/23/2023.
Data uploaded by NhuBui and last updated on 2024-12-20 20:54:27.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Rosetta Score for W325A: 6.957

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.