Raw Data for BglB Variant R151A

Expression Data

Yield (mg/mL): 5.612

Kinetic Assay Data

slope of R151A A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00		0.0061	0.00525
	B	25.00		0.0045	0.00535
	C	8.33		0.00335	0.00395
	D	2.78		0.00335	0.0027
	E	0.93		0.00155	0.00145
	F	0.31	0.0011	0.00105
	G	0.10		0.00085	0.0007
	H	0.03	0	0

Yield (mg/mL): 5.612     Dilution Factor:  100×

Download these data

Protein purified on 05/17/2023 and assayed on 05/24/2023.
Data uploaded by YuanAngela666 and last updated on 2023-05-31 10:18:11.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of R151A A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.052	0.048	0.05
	B	48.3	0.054	0.051	0.051
	C	45.7	0.05	0.051	0.05
	D	42.4	0.055	0.049	0.051
	E	37.7	0.056	0.051	0.052
	F	33.6	0.051	0.05	0.049
	G	31.3	0.053	0.052	0.048
	H	30.0	0.056	0.055	0.049

Download these data

Protein purified on 05/17/2023 and assayed on 05/24/2023.
Data uploaded by YuanAngela666 and last updated on 2023-05-31 10:56:08.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.