Raw Data for BglB Variant I91R
Expression Data
0.156
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 5.44E-02 | 5.38E-02 | 5.45E-02 | ||
| B | 25.00 | 4.35E-02 | 4.37E-02 | 4.32E-02 | ||
| C | 8.33 | 3.11E-02 | 3.30E-02 | 3.19E-02 | ||
| D | 2.78 | 1.70E-02 | 1.77E-02 | 2.07E-02 | ||
| E | 0.93 | 7.95E-03 | 8.50E-03 | 7.95E-03 | ||
| F | 0.31 | 3.25E-03 | 3.20E-03 | |||
| G | 0.10 | 1.40E-03 | 1.25E-03 | 1.25E-03 | ||
| H | 0.03 | 2.00E-04 | 2.00E-04 |
Protein purified on 4/27/2023 and assayed on 5/6/2023.
Data uploaded by cajbio204 and last updated on 2023-05-18 11:30:06.
Reference Data for WT
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 5.06E-02 | 4.93E-02 | 4.98E-02 | ||
| B | 25.00 | 3.93E-02 | 3.87E-02 | 3.85E-02 | ||
| C | 8.33 | 3.19E-02 | 3.02E-02 | 3.21E-02 | ||
| D | 2.78 | 2.04E-02 | 1.82E-02 | 1.70E-02 | ||
| E | 0.93 | 6.65E-03 | 6.75E-03 | 7.00E-03 | ||
| F | 0.31 | 2.90E-03 | 2.55E-03 | 2.70E-03 | ||
| G | 0.10 | 1.00E-03 | 1.25E-03 | 1.05E-03 | ||
| H | 0.03 | 2.50E-04 | 1.50E-04 | 0.00E+00 |
Protein purified on 4/27/2023 and assayed on 5/6/2023.
Data uploaded by ToniB and last updated on 2023-05-18 11:26:43.
Thermostability Assay Data
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 0.00E+00 | 0.00E+00 | |||
| B | 48.3 | 2.00E-04 | 2.50E-04 | |||
| C | 45.7 | 2.50E-04 | 2.50E-04 | 2.50E-04 | ||
| D | 42.4 | 2.10E-03 | 1.85E-03 | 1.75E-03 | ||
| E | 37.7 | 2.02E-02 | 1.96E-02 | 1.92E-02 | ||
| F | 33.6 | 2.50E-02 | 2.64E-02 | 2.45E-02 | ||
| G | 31.3 | 2.76E-02 | 2.51E-02 | 2.49E-02 | ||
| H | 30.0 | 2.61E-02 | 2.32E-02 | 2.27E-02 |
Reference Data for WT
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 3.00E-04 | 2.50E-04 | 1.50E-04 | ||
| B | 48.3 | 2.50E-04 | 2.50E-04 | |||
| C | 45.7 | 1.10E-03 | 7.50E-04 | 5.50E-04 | ||
| D | 42.4 | 9.00E-03 | 8.00E-03 | 8.95E-03 | ||
| E | 37.7 | 2.72E-02 | 2.77E-02 | 2.71E-02 | ||
| F | 33.6 | 3.15E-02 | 3.26E-02 | 2.93E-02 | ||
| G | 31.3 | 3.38E-02 | 3.48E-02 | 3.22E-02 | ||
| H | 30.0 | 3.52E-02 | 3.53E-02 | 3.36E-02 |
Protein purified on 4/27/2023 and assayed on 5/8/2023.
Data uploaded by tolsy2000 and last updated on 2023-05-18 11:38:46.
Design Data
Citations
The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.