Raw Data for BglB Variant I91R
Expression Data
0.156
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 1.16E-02 | 1.16E-02 | 1.12E-02 | ||
| B | 25.00 | 9.50E-03 | 9.45E-03 | 9.35E-03 | ||
| C | 8.33 | 6.85E-03 | 7.20E-03 | 8.05E-03 | ||
| D | 2.78 | 4.00E-03 | 3.90E-03 | 4.35E-03 | ||
| E | 0.93 | 2.20E-03 | 1.90E-03 | 1.95E-03 | ||
| F | 0.31 | 9.50E-04 | 9.50E-04 | |||
| G | 0.10 | 5.00E-04 | 4.00E-04 | 5.00E-04 | ||
| H | 0.03 | 2.50E-04 | 2.50E-04 |
Protein purified on 4/17/23 and assayed on 5/6/23.
Data uploaded by lopezalexis and last updated on 2023-12-20 09:02:31.
Reference Data for WT
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 7.38E-02 | 7.57E-02 | 7.24E-02 | ||
| B | 25.00 | 6.23E-02 | 6.03E-02 | 5.61E-02 | ||
| C | 8.33 | 4.70E-02 | 4.69E-02 | 4.26E-02 | ||
| D | 2.78 | 2.55E-02 | 2.71E-02 | 2.58E-02 | ||
| E | 0.93 | 1.22E-02 | 1.13E-02 | 1.16E-02 | ||
| F | 0.31 | 4.40E-03 | 4.30E-03 | 4.60E-03 | ||
| G | 0.10 | 1.70E-03 | 1.60E-03 | |||
| H | 0.03 | 0.00E+00 | 0.00E+00 |
Protein purified on 2/7/23 and assayed on 5/6/23.
Data uploaded by delaneycarpenter and last updated on 2023-05-18 11:14:16.
Thermostability Assay Data
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 2.00E-04 | 2.50E-04 | |||
| B | 48.3 | 0.00E+00 | 0.00E+00 | 0.00E+00 | ||
| C | 45.7 | 2.50E-04 | 3.00E-04 | 2.00E-04 | ||
| D | 42.4 | 3.00E-04 | 3.00E-04 | |||
| E | 37.7 | 9.00E-04 | 1.40E-03 | 1.05E-03 | ||
| F | 33.6 | 1.25E-03 | 1.40E-03 | 1.30E-03 | ||
| G | 31.3 | 1.30E-03 | 1.80E-03 | 1.55E-03 | ||
| H | 30.0 | 1.40E-03 | 1.25E-03 | 1.35E-03 |
Reference Data for WT
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 3.00E-04 | 2.50E-04 | 1.50E-04 | ||
| B | 48.3 | 2.50E-04 | 2.50E-04 | |||
| C | 45.7 | 1.10E-03 | 7.50E-04 | 5.50E-04 | ||
| D | 42.4 | 9.00E-03 | 8.00E-03 | 8.95E-03 | ||
| E | 37.7 | 2.72E-02 | 2.77E-02 | 2.71E-02 | ||
| F | 33.6 | 3.15E-02 | 3.26E-02 | 2.93E-02 | ||
| G | 31.3 | 3.38E-02 | 3.48E-02 | 3.22E-02 | ||
| H | 30.0 | 3.52E-02 | 3.53E-02 | 3.36E-02 |
Protein purified on 4/27/2023 and assayed on 5/8/2023.
Data uploaded by tolsy2000 and last updated on 2023-05-18 11:38:46.
Design Data
Citations
The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.