Raw Data for BglB Variant I91A

Expression Data

Yield (mg/mL): 0.103

Kinetic Assay Data

slope of I91A A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.38E-01	2.54E-01	2.41E-01
	B	25.00	1.93E-01	1.97E-01	1.98E-01
	C	8.33	1.61E-01	1.49E-01	1.53E-01
	D	2.78	8.60E-02	8.37E-02	8.13E-02
	E	0.93	4.17E-02	3.70E-02	3.66E-02
	F	0.31	1.53E-02	1.55E-02	1.27E-02
	G	0.10	5.50E-03	4.90E-03	4.65E-03
	H	0.03		0.00E+00	0.00E+00

Yield / A_{280 nm}: 0.103     Dilution Factor:  10×

Download these data

Protein purified on 4/27/23 and assayed on 5/6/23.
Data uploaded by marianaa and last updated on 2023-06-01 10:26:34.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of I91A A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0		0
	B	48.3	3.00E-04	2.50E-04	2.50E-04
	C	45.7	4.00E-04	3.50E-04	4.00E-04
	D	42.4	7.65E-03	7.35E-03	5.80E-03
	E	37.7	1.21E-01	1.06E-01	1.12E-01
	F	33.6	1.26E-01	1.28E-01	1.23E-01
	G	31.3	1.29E-01	1.22E-01	1.26E-01
	H	50.0	1.24E-01	1.32E-01	1.27E-01

Download these data

Protein purified on 4/27/23 and assayed on 5/8/23.
Data uploaded by jabates004 and last updated on 2023-05-18 11:42:42.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.