D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I106A

Expression Data

Yield (mg/mL): 1.029

Kinetic Assay Data

**slope of I106A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.16E-01	2.01E-01	1.99E-01
B	25.00	1.84E-01	2.08E-01	1.84E-01
C	8.33	1.28E-01	1.43E-01	1.33E-01
D	2.78	7.83E-02	7.66E-02	7.91E-02
E	0.93	3.58E-02	3.46E-02	3.54E-02
F	0.31	1.27E-02	1.27E-02	1.38E-02
G	0.10		2.03E-03	1.89E-03
H	0.03	0.00E+00	0.00E+00	0.00E+00

Yield (mg/mL): 1.029 Dilution Factor: 100×

Protein purified on 4/12/2023 and assayed on 4/14/2023.
Data uploaded by Augustagarr and last updated on 2023-05-03 23:15:01.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.35E-01	2.21E-01	2.03E-01
B	25.00	2.03E-01	2.16E-01	1.91E-01
C	8.33	1.63E-01	1.57E-01	1.64E-01
D	2.78	9.10E-02	9.20E-02	9.54E-02
E	0.93	3.96E-02	3.97E-02	4.38E-02
F	0.31	1.26E-02	1.38E-02	1.57E-02
G	0.10	2.64E-03	2.48E-03	3.01E-03
H	0.03

Yield (mg/mL): 1.1888 Dilution Factor: 100×

Protein purified on 4/12/2023 and assayed on 4/14/2023.
Data uploaded by cwolfe and last updated on 2023-04-26 12:52:53.

Citations

The unpublished data above were collected by students from Kentucky Wesleyan College. For inquiries, please contact Cindy Wolfe: <cindy.wolfe@kwc.edu>.