Raw Data for BglB Variant Y375H

Expression Data

Kinetic Assay Data

Plate 20201217-2

slope of Y375H A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.262147	0.277404	0.258862
	B	18.75	0.205273	0.207927	0.200396
	C	4.69	0.126744	0.129346	0.124392
	D	1.17	0.059697	0.0602806	0.0577576
	E	0.29	0.0195061	0.0204048	0.0197473
	F	0.07	0.00542121	0.00568485	0.00539152
	G	0.02	0.00143394	0.00151212	0.0014
	H	0.00		0	0

Yield / A_{280 nm}: 0.493     Dilution Factor:  10×

Download these data

Protein purified on 9/21/19 and assayed on 9/21/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	8.86E-02	1.11E-01	8.31E-02
	B	18.75	7.30E-02	6.36E-02	5.65E-02
	C	4.69	5.06E-02	4.93E-02	4.65E-02
	D	1.17	2.51E-02	2.37E-02	2.22E-02
	E	0.29	9.62E-03	8.33E-03	8.61E-03
	F	0.07	2.82E-03	2.38E-03	2.40E-03
	G	0.02	7.90E-04	6.74E-04	7.26E-04
	H	0.00	1.88E-05	4.73E-05	0

Yield / A_{280 nm}: 1.03     Dilution Factor:  100×

Download these data

Protein purified on 9/21/19 and assayed on 9/21/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Citations

The above data have been published in the following journal article(s):

• • Huang, P.; Chu, S.K.; Frizzo, H.N.; Connolly, M.P.; Caster, R.W.; & Siegel, J. B. (2020) “Evaluating Protein Engineering Thermostability Prediction Tools Using an Independently Generated Dataset..” ACS Omega 5(12), 6487–6493.