Raw Data for BglB Variant N220S

Expression Data

Kinetic Assay Data

slope of N220S A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.03E-02	1.98E-02	1.80E-02
	B	25.00	1.25E-02	1.20E-02	1.22E-02
	C	8.33	6.00E-03	5.85E-03	5.85E-03
	D	2.78	2.70E-03	2.70E-03	2.55E-03
	E	0.93	1.20E-03	1.20E-03	1.20E-03
	F	0.31		6.00E-04	9.00E-04
	G	0.10	9.00E-04	1.20E-03	9.00E-04
	H	0.03	6.00E-04	6.00E-04	6.00E-04

Yield (mg/mL): 1.238     Dilution Factor:  10×

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Protein purified on and assayed on .
Data uploaded by mayoral and last updated on 2023-04-26 14:19:36.

Thermostability Assay Data

slope of N220S A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0	0	0
	B	48.3	0	0	0
	C	45.7	1.20E-03		1.20E-03
	D	42.4	3.21E-02	3.39E-02	3.21E-02
	E	37.7	8.52E-02	7.68E-02	7.83E-02
	F	33.6	9.36E-02	8.16E-02	8.55E-02
	G	31.3	9.21E-02	9.03E-02	8.55E-02
	H	30.0	8.64E-02	9.66E-02	9.27E-02

Download these data

Protein purified on and assayed on .
Data uploaded by mayoral and last updated on 2023-04-26 14:26:03.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.