D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E154F

Expression Data

Yield (mg/mL): 0.721

Kinetic Assay Data

**slope of E154F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		5.03E-01	4.59E-01
B	25.00	3.83E-01	3.61E-01	3.92E-01
C	8.33	3.05E-01	2.55E-01	2.57E-01
D	2.78	1.77E-01	1.59E-01	1.65E-01
E	0.93	6.32E-02	6.37E-02	6.10E-02
F	0.31	3.09E-02	2.69E-02	2.06E-02
G	0.10	1.02E-02	8.80E-03	9.33E-03
H	0.03

Yield (mg/mL): 0.721 Dilution Factor: 100×

Protein purified on 4/12/23 and assayed on 4/14/23.
Data uploaded by Thayes and last updated on 2023-04-26 14:40:51.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.35E-01	2.21E-01	2.03E-01
B	25.00	2.03E-01	2.16E-01	1.91E-01
C	8.33	1.63E-01	1.57E-01	1.64E-01
D	2.78	9.10E-02	9.20E-02	9.54E-02
E	0.93	3.96E-02	3.97E-02	4.38E-02
F	0.31	1.26E-02	1.38E-02	1.57E-02
G	0.10	2.64E-03	2.48E-03	3.01E-03
H	0.03

Yield (mg/mL): 1.1888 Dilution Factor: 100×

Protein purified on 4/12/2023 and assayed on 4/14/2023.
Data uploaded by cwolfe and last updated on 2023-04-26 12:52:53.

Citations

The unpublished data above were collected by students from Kentucky Wesleyan College. For inquiries, please contact Cindy Wolfe: <cindy.wolfe@kwc.edu>.