Raw Data for BglB Variant H315K

Expression Data

Kinetic Assay Data

Plate 20201217-2

slope of H315K A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.0185467	0.0189455	0.0161758
	B	18.75	0.0139933	0.0142291	0.0124897
	C	4.69	0.00832121	0.0088697	0.00768121
	D	1.17	0.00353939	0.00365212	0.00330424
	E	0.29	0.0010097	0.00111091	0.000975758
	F	0.07	0.000285455	0.000326061	0.000307273
	G	0.02	0.000132727		0.000132121
	H	0.00	5.94E-05	1.38E-04	0

Yield / A_{280 nm}: 0.384     Dilution Factor:  10×

Download these data

Protein purified on 9/21/19 and assayed on 9/21/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	8.86E-02	1.11E-01	8.31E-02
	B	18.75	7.30E-02	6.36E-02	5.65E-02
	C	4.69	5.06E-02	4.93E-02	4.65E-02
	D	1.17	2.51E-02	2.37E-02	2.22E-02
	E	0.29	9.62E-03	8.33E-03	8.61E-03
	F	0.07	2.82E-03	2.38E-03	2.40E-03
	G	0.02	7.90E-04	6.74E-04	7.26E-04
	H	0.00	1.88E-05	4.73E-05	0

Yield / A_{280 nm}: 1.03     Dilution Factor:  100×

Download these data

Protein purified on 9/21/19 and assayed on 9/21/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Citations

The above data have been published in the following journal article(s):

• • Huang, P.; Chu, S.K.; Frizzo, H.N.; Connolly, M.P.; Caster, R.W.; & Siegel, J. B. (2020) “Evaluating Protein Engineering Thermostability Prediction Tools Using an Independently Generated Dataset..” ACS Omega 5(12), 6487–6493.