Raw Data for BglB Variant H194I

Expression Data

SDS-PAGE
 0.555

Kinetic Assay Data

slope of H194I A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.74E-01 1.83E-01 1.68E-01
B 25.00 1.42E-01 1.58E-01 1.40E-01
C 8.33 1.06E-01 1.08E-01 1.06E-01
D 2.78 6.04E-02 6.12E-02 5.62E-02
E 0.93 2.55E-02 2.52E-02 2.50E-02
F 0.31 8.95E-03 9.35E-03 1.02E-02
G 0.10 3.20E-03 3.20E-03 3.85E-03
H 0.03 0 0 2.00E-04
 0.555      10×

Protein purified on 3/27/2023 and assayed on 3/31/2023.
Data uploaded by Mahnoor and last updated on 2023-04-17 16:53:39.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 5.18E-02 5.52E-02 5.54E-02
B 25.00 4.95E-02 5.28E-02 5.38E-02
C 8.33 3.67E-02 3.83E-02 3.82E-02
D 2.78 2.11E-02 2.67E-02 2.21E-02
E 0.93 8.50E-03 1.05E-02 1.05E-02
F 0.31 3.35E-03 4.25E-03
G 0.10 1.30E-03 1.45E-03
H 0.03 2.00E-04 0 2.50E-04
 1.556      100×

Protein purified on 3/27/2023 and assayed on 3/31/2023.
Data uploaded by Mahnoor and last updated on 2023-04-17 17:00:45.


Thermostability Assay Data

slope of H194I A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 8.30E-03 5.30E-03
B 48.8 5.00E-04 6.00E-04
C 46.5 8.80E-03 0 5.00E-04
D 42.5 5.97E-02 7.35E-02 6.79E-02
E 37.6 8.17E-02 8.88E-02
F 33.8 9.01E-02 8.34E-02
G 31.2 9.37E-02 1.05E-01 1.07E-01
H 30.0 8.89E-02 1.07E-01 9.32E-02

Protein purified on 3/27/2023 and assayed on 4/3/2023.
Data uploaded by Mahnoor and last updated on 2023-04-17 17:16:14.


Reference Data for WT

slope of H194I A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 5.00E-04 5.00E-04
B 48.8 6.00E-04 0 4.00E-04
C 46.5 1.00E-03 1.20E-03 7.00E-04
D 42.5 9.85E-02 9.03E-02 9.18E-02
E 37.6 6.03E-02 6.48E-02
F 33.8 9.21E-02 1.00E-01 8.46E-02
G 31.2 9.85E-02 8.43E-02 1.01E-01
H 30.0 9.26E-02 9.86E-02 1.01E-01

Protein purified on 3/27/2023 and assayed on 4/3/2023.
Data uploaded by Mahnoor and last updated on 2023-04-17 17:18:48.


Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Linda Brzezinski: <brzezinskil@yosemite.edu>.