D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Q124E

Expression Data

Yield (mg/mL): 0.48

Kinetic Assay Data

**slope of Q124E A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.20E-03	5.85E-03	7.25E-03
B	25.00	5.15E-03	6.75E-03	5.55E-03
C	8.33	5.45E-03	4.30E-03	3.30E-03
D	2.78	2.60E-03		2.50E-03
E	0.93	1.60E-03	1.60E-03
F	0.31	7.50E-04	8.50E-04	6.00E-04
G	0.10		4.50E-04	4.00E-04
H	0.03	3.00E-04	0	2.50E-04

Yield (mg/mL): 0.48 Dilution Factor: 100×

Protein purified on 3/27/2023 and assayed on 3/31/2023.
Data uploaded by jayden921458 and last updated on 2023-04-17 16:57:18.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	7.15E-02	7.22E-02	7.17E-02
B	25.00	5.55E-02	6.19E-02	5.91E-02
C	8.33	4.44E-02	4.47E-02	4.70E-02
D	2.78	3.04E-02	2.80E-02	3.42E-02
E	0.93	1.72E-02	1.31E-02	1.78E-02
F	0.31	6.95E-03	5.20E-03	7.90E-03
G	0.10	2.50E-03	2.10E-03	2.75E-03
H	0.03	0	2.00E-04	0

Yield (mg/mL): 2.41 Dilution Factor: 100×

Protein purified on 3/27/2023 and assayed on 3/31/2023.
Data uploaded by jayden921458 and last updated on 2023-04-17 17:02:00.

Thermostability Assay Data

**slope of Q124E A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	8.00E-04	6.00E-04	5.00E-04
B	48.8	6.00E-04		6.00E-04
C	46.5	8.00E-04	8.00E-04	8.00E-04
D	42.5	3.20E-03	3.40E-03
E	37.6	4.27E-02	4.27E-02	3.36E-02
F	33.8	6.82E-02	6.66E-02	6.14E-02
G	31.2	7.65E-02	8.11E-02	9.18E-02
H	30.0	1.04E-01	1.01E-01	1.07E-01

Protein purified on 3/27/2023 and assayed on 4/3/2023.
Data uploaded by jayden921458 and last updated on 2023-04-17 17:31:58.

Reference Data for WT

**slope of Q124E A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0.001500	0.001000	0
B	48.8	0	0.0005000	0.0008000
C	46.5	0.001200	0.001000
D	42.5	0.03320	0.02810	0.02460
E	37.6	0.08170	0.06340	0.07460
F	33.8	0.09360	0.08560	0.07760
G	31.2		0.08079	0.08150
H	30.0	0.1009	0.08870	0.09760

Protein purified on 3/27/2023 and assayed on 4/3/2023.
Data uploaded by jayden921458 and last updated on 2023-04-17 17:31:06.

Design Data

Rosetta Score for Q124E: 5.559

Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Linda Brzezinski: <brzezinskil@yosemite.edu>.