Raw Data for BglB Variant Q124M

Expression Data

SDS-PAGE
 0.54

Kinetic Assay Data

slope of Q124M A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.01E-02 1.82E-02 1.58E-02
B 25.00 1.66E-02 1.86E-02 1.71E-02
C 8.33 1.44E-02 1.30E-02 1.31E-02
D 2.78 1.03E-02 8.45E-03 8.00E-03
E 0.93 3.95E-03 4.25E-03 4.20E-03
F 0.31 1.65E-03 1.75E-03 1.80E-03
G 0.10 7.00E-04 7.50E-04 8.00E-04
H 0.03 0 0 0
 0.54      100×

Protein purified on 3/27/2023 and assayed on 3/31/2023.
Data uploaded by drvl1103 and last updated on 2023-04-17 16:49:58.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 7.15E-02 7.22E-02 7.17E-02
B 25.00 5.55E-02 6.19E-02 5.91E-02
C 8.33 4.44E-02 4.47E-02 4.70E-02
D 2.78 3.04E-02 2.80E-02 3.42E-02
E 0.93 1.72E-02 1.31E-02 1.78E-02
F 0.31 6.95E-03 5.20E-03 7.90E-03
G 0.10 2.50E-03 2.10E-03 2.75E-03
H 0.03 0 2.00E-04 0
 2.41      100×

Protein purified on 3/27/2023 and assayed on 3/31/2023.
Data uploaded by jayden921458 and last updated on 2023-04-17 17:02:00.


Thermostability Assay Data

slope of Q124M A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0009000 0.0009000
B 48.8 0.0006001 0.0007000
C 46.5 0.0007000 0.001100 0.0009000
D 42.5 0.005600 0.005400 0.007000
E 37.6 0.1231 0.1268 0.1321
F 33.8 0.1856 0.1757 0.1873
G 31.2 0.1815 0.1791 0.1925
H 30.0 0.1825 0.1686 0.1824

Protein purified on 3/27/2023 and assayed on 4/3/2023.
Data uploaded by drvl1103 and last updated on 2023-04-17 17:16:54.


Reference Data for WT

slope of Q124M A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.001500 0.001000 0
B 48.8 0 0.0005000 0.0008000
C 46.5 0.001200 0.001000
D 42.5 0.03320 0.02810 0.02460
E 37.6 0.08170 0.06340 0.07460
F 33.8 0.09360 0.08560 0.07760
G 31.2 0.08079 0.08150
H 30.0 0.1009 0.08870 0.09760

Protein purified on 3/27/2023 and assayed on 4/3/2023.
Data uploaded by jayden921458 and last updated on 2023-04-17 17:31:06.


Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Linda Brzezinski: <brzezinskil@yosemite.edu>.