Raw Data for BglB Variant H194Y

Expression Data

 0.76

Kinetic Assay Data

slope of H194Y A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 5.04E-02 3.54E-02 3.80E-02
B 25.00 2.78E-02 3.12E-02 3.28E-02
C 8.33 2.52E-02 2.16E-02 2.39E-02
D 2.78 1.35E-02 1.56E-02 1.38E-02
E 0.93 5.85E-03 6.05E-03 6.45E-03
F 0.31 2.65E-03 2.60E-03 2.70E-03
G 0.10 1.20E-03 1.00E-03 1.05E-03
H 0.03 0 2.00E-04 4.50E-04
 0.76      100×

Protein purified on 3/27/2023 and assayed on 3/31/2023.
Data uploaded by Bubbles and last updated on 2023-04-17 16:51:10.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.43E-02 3.41E-02
B 25.00 3.38E-02 3.30E-02 3.37E-02
C 8.33 2.62E-02 2.68E-02 2.51E-02
D 2.78 1.53E-02 1.58E-02 1.58E-02
E 0.93 7.00E-03 6.75E-03 7.20E-03
F 0.31 2.65E-03 2.60E-03 2.80E-03
G 0.10 1.10E-03 1.10E-03 1.10E-03
H 0.03 4.00E-04 0 2.50E-04
 0.72      100×

Protein purified on 3/27/2023 and assayed on 3/31/2023.
Data uploaded by Niconicolas and last updated on 2023-04-17 16:49:50.


Thermostability Assay Data

slope of H194Y A420 nm (min−1)
T (°C) 1 2 3
A 50.0 1.50E-03 7.00E-04
B 48.8 1.20E-03 0 9.00E-04
C 46.5 7.00E-04 1.50E-03
D 42.5 1.00E-02 8.70E-03
E 37.6 1.57E-01 1.72E-01 1.50E-01
F 33.8 1.94E-01 1.87E-01 1.98E-01
G 31.2 2.07E-01 2.07E-01 2.24E-01
H 30.0 2.08E-01 2.30E-01 2.18E-01

Protein purified on 3/27/2023 and assayed on 4/3/2023.
Data uploaded by Bubbles and last updated on 2023-04-17 17:12:51.


Reference Data for WT

slope of H194Y A420 nm (min−1)
T (°C) 1 2 3
A 50.0 5.00E-04 5.00E-04
B 48.8 3.00E-04 4.00E-04
C 46.5 1.10E-03 1.00E-03 9.00E-04
D 42.5 5.14E-02 4.76E-02
E 37.6 2.13E-01 1.84E-01 1.86E-01
F 33.8 1.93E-01 1.97E-01 1.98E-01
G 31.2 1.99E-01 2.05E-01
H 30.0 1.91E-01 2.05E-01 1.96E-01

Protein purified on 3/27/2023 and assayed on 4/3/2023.
Data uploaded by Niconicolas and last updated on 2023-04-17 17:13:35.


Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Linda Brzezinski: <brzezinskil@yosemite.edu>.