D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I387K

Expression Data

Yield (mg/mL): 0.165

Kinetic Assay Data

**slope of I387K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.83E-02	4.15E-02	3.87E-02
B	25.00	3.16E-02	3.10E-02	3.11E-02
C	8.33	2.31E-02	2.05E-02	2.08E-02
D	2.78	1.20E-02	1.18E-02	1.26E-02
E	0.93	5.92E-03	5.95E-03	5.63E-03
F	0.31	2.16E-03	2.75E-03	2.29E-03
G	0.10	9.07E-04	8.45E-04	9.02E-04
H	0.03	2.01E-04	2.35E-04	2.58E-04

Yield (mg/mL): 0.165 Dilution Factor: 10×

Protein purified on 4/13/2023 and assayed on 4/14/2023.
Data uploaded by kasiapno and last updated on 2023-04-17 16:35:30.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.90E-02	8.91E-02	7.56E-02
B	25.00	7.95E-02	7.43E-02	7.78E-02
C	8.33	6.00E-02	5.31E-02	5.09E-02
D	2.78	2.99E-02	2.85E-02	2.73E-02
E	0.93	1.42E-02	1.44E-02	1.25E-02
F	0.31	5.18E-03	6.47E-03	5.69E-03
G	0.10	1.81E-03	2.01E-03	2.05E-03
H	0.03	1.32E-04	1.19E-04

Yield (mg/mL): 0.777 Dilution Factor: 100×

Protein purified on 4/13/2023 and assayed on 4/14/2023.
Data uploaded by kasiapno and last updated on 2023-04-17 16:25:44.

Design Data

Rosetta Score for I387K: 4.685

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.