Raw Data for BglB Variant Y396W

Expression Data

SDS-PAGE
 1.281

Kinetic Assay Data

slope of Y396W A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.17E-02 6.09E-02
B 25.00
C 8.33 4.70E-02 5.52E-02 4.52E-02
D 2.78 3.35E-02 3.50E-02 3.05E-02
E 0.93 1.50E-02 1.34E-02 1.11E-02
F 0.31 5.55E-03 4.35E-03 5.40E-03
G 0.10 2.10E-03 2.25E-03
H 0.03 0 0 0
 1.281      10×

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by BrandenRuiz037 and last updated on 2023-04-14 12:08:13.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 8.21E-02 5.46E-02
B 25.00 6.29E-02 5.49E-02
C 8.33 5.96E-02 4.01E-02 5.10E-02
D 2.78 2.87E-02 2.43E-02
E 0.93 9.60E-03 1.40E-02 1.68E-02
F 0.31 5.85E-03 5.70E-03
G 0.10 2.40E-03 2.85E-03 2.85E-03
H 0.03 6.00E-04 7.50E-04 0
 1.159      100×

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by sabrinamansoor and last updated on 2023-04-18 09:38:49.


Thermostability Assay Data

slope of Y396W A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 6.00E-04 7.50E-04
B 48.3 9.00E-04 0 0
C 45.7 6.00E-04 9.00E-04 0
D 42.4 1.20E-03 1.20E-03
E 37.7 1.83E-02 1.55E-02 1.67E-02
F 33.6 2.43E-02 2.22E-02 1.88E-02
G 31.3 2.57E-02 2.52E-02 2.75E-02
H 30.0 2.04E-02 1.85E-02 2.21E-02

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by BrandenRuiz037 and last updated on 2023-04-18 09:36:28.


Reference Data for WT

slope of Y396W A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 4.50E-04
B 48.3 7.50E-04
C 45.7 6.00E-04 7.50E-04 7.50E-04
D 42.4 1.20E-02 1.34E-02 1.22E-02
E 37.7 3.12E-01 2.94E-01 2.77E-01
F 33.6 2.60E-01 2.72E-01 3.01E-01
G 31.3 3.38E-01 3.54E-01 3.54E-01
H 30.0 3.17E-01 3.00E-01 3.08E-01

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by sabrinamansoor and last updated on 2023-04-18 09:38:20.


Design Data

3.928

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.